Inhibitory effect of a propolis on Di-n-Propyl Disulfide or n-Hexyl salycilate-induced skin irritation, oxidative stress and inflammatory responses in mice
Nada
Oršolića, Jadranka Skurićb, Domagoj Đikića, Gabrijela Stanićc
Department
of Animal Physiology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Clinic
of Anaesthesia and Intensive Care Unit, Sveti Duh General Hospital, Zagreb,
Croatia
Department
of Pathology, Sveti Duh General Hospital, Zagreb, Croatia
Purpose
Thermal imaging has
been utilised, both preclinically and clinically, as a tool for assessing inflammation.
Psoriasis is a chronic inflammatory skin disease characterised by
hyperkeratosis, dermal inflammatory infiltrate and increased angiogenesis. The
aim of the present study was to assess the usefulness of thermography in
psoriatic lesion regression after topical treatment with bee propolis,
recognised as potent antioxidants and anti-inflammatory agents.
Methods
We monitored the
inflammation process induced by irritants such as n-Hexyl salycilate (HXS) or
Di-n-Propyl Disulfide (PPD) by histopatological assessment of the skin,
thermographic scanning, total number of inflammatory cells in the peritoneal
cavity, differential analysis of cells in the peritoneal cavity, macrophage
spreading index, haematological and biochemical parameters, frequencies of micronucleated
reticulocytes, lipid peroxidation and glutathione assay in the skin.
Results
Topically applied
ethanolic extract of propolis (EEP) with HXS or PPD reduced the lipid
peroxidation in the skin and total number of inflammatory cells in the skin and
peritoneal cavity, functional activity of macrophages, the number of
micronuclei in mouse peripheral blood reticulocytes and enzymatic activity of
ALP and AST.
Conclusion
These results
demonstrate that topical application of EEP may improve psoriatic-like skin
lesions by suppressing functional activity of macrophages and ROS production.
Taken together, it is suggested that EEP can safely be utilised in the
prevention of psoriasis-related inflammatory changes without causing any toxic
effect.
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